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KMID : 0380620210530020204
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Korean Journal of Food Science and Technology
2021 Volume.53 No. 2 p.204 ~ p.212
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Chemical changes in resazurin by probiotics and its application for evaluating living bacterial cell counts and their reduction potentials
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Lee Hyo-Won
Oh Yeong-Ji
Hong Jung-Il
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Abstract
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Resazurin, an oxidized blue dye, is reduced to resorufin, showing a peak absorbance change and emitting fluorescence due to the metabolic activity of living cells. In this study, the growth of Lactobacillus rhamnosus GG (LGG) and the redox potential of living probiotic bacteria were evaluated based on changes in the absorbance or fluorescence of resazurin. Fluorescence analysis is a more accurate and sensitive method for quantifying viable LGG than the colorimetric absorbance measurement of resazurin change. Fluorescence measurement could detect LGG of 6.5-9.5 log CFU/mL within 30 min with R2=0.99. No significant effect of further reduction of resorufin to dihydroresorufin by LGG was observed.
Various probiotics showed different resazurin-reducing activities, and L. kimchicus had the highest reducing activity among the six probiotics tested. These findings suggests that fluorescence measurement in a resazurin-based assay is useful for analyzing bacterial growth and the redox potential of living probiotics.
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KEYWORD
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resazurin, probiotics, resorufin, Lactobacillus rhamnosus GG, Lactobacillus kimchicus
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